ARAP-PLUS
allows integral and selective determination of
the anti-radical ability of substances: ACW -
anti-radical capacity of water-soluble substances
(it is similar to: TRAP – "total radical-trapping
antioxidant potential", TAS - "total
antioxidant status", ORAC – "oxygen
radical absorbance capacity" etc.), ACU -
urate-independent component of ACW and ASC - anti-radical
capacity of ascorbic acid.
Description
ARAP-PLUS
Biochemical
basis
Ability
of substances to react with free radicals
Measuring
principle
based
on the chemiluminescence detection of free
radicals which are generated during thermo-initiated
breaking of an azo-compound. Changes of
parameters of the signal are evaluated with
respect to a calibration substance.
KIT components
• for each measurement
one ampoule
• calibration substance
• buffer
• enzyme solution
Features
•
ready-to-use, easy one-step operation
• duration of measurement well under
2 minutes
• within- and between-batch variabilities
< 2 %
• very low demand for sample volumes
(i.e. 5 µl plasma,
20 µl of liquids
like tea, juices diluted 1:100 ... 1:1000)
• works with any luminometer having
a thermostated measuring cell
• suitable for automation
Applications
ARAP-PLUS
Medical
all
fields involving free radicals/antioxidants
Industrial
monitoring
and optimization of antioxidant dependent
processes (preparation, storage), screening
for new substances with anti-radical properties,
detection of food irradiation etc.
