ARAP is a KIT for characterization of oxidative
stress using the anti-radical ability of proteins
as a marker.
Description
ARAP
Biochemical
basis
Discovery
that at least seven constituents of proteins
(cystine, histidine, methionine, phenylalanine,
serine, tryptophan, and tyrosine) acquire
antiradical capacity after oxidative modification
(Popov/Lewin, 1996).
Measuring
principle
based
on the chemiluminescence detection of free
radicals which are generated during thermo-initiated
breaking of an azo-compound. Changes of
parameters of the signal are evaluated with
respect to a calibration substance.
KIT components
• for each measurement
one ampoule
• calibration substance
• buffer
• desalting columns for sample preparation
Features
•
ready-to-use
• duration of measurement well under
2 minutes
• within- and between-batch variabilities
< 2 %
• very low demand for sample volumes
(i.e. 50 µl blood plasma)
• works with any luminometer having
a thermostated measuring cell
• suitable for automation and microtiter
Applications
ARAP
Warning signal
•
preventive medicine
• sports medicine (i.e. detection
of overtraining)
Curative medicine
•
follow-up and prognosis of complications
• control of dosage of antioxidative
therapies
